To detect the presence of herpes virus in corneal scrapings/corneal grafts of suspected herpetic keratitis patients attending the outpatient department/casualty of the Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi for the past 20 years with immunofluorescence assay and to analyse the efficacy of polymerase chain reaction over immunofluorescence for routine laboratory diagnosis in some of the specimens.

Immunofluorescence and polymerase chain reaction are effective methods for detecting ocular herpes virus in corneal scrapings and grafts, with PCR being more effective for routine laboratory diagnosis.

Paper

A 20‐year experience of ocular herpes virus detection using immunofluorescence and polymerase chain reaction

References

Citations